Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

A high-throughput assay for assessing the cell permeability of combinatorial libraries

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Yu, P.
  • Liu, B.
  • Kodadek, Thomas

publication date

  • June 2005

journal

  • Nature Biotechnology  Journal

abstract

  • There is great interest in the identification of synthetic molecules that are capable of manipulating protein-protein interactions in living cells. Peptides, unlike other classes of small molecules, have binding properties appropriate for this application, but most are poorly cell permeable and sensitive to proteases. Therefore, considerable effort has been expended in the development of libraries of oligomeric peptide-like molecules. However, there are no clear-cut rules to guide the design of libraries rich in cell permeable compounds. Furthermore, currently available empirical methods to assess permeability may not accurately reflect true permeability and/or are capable of only modest throughput. We describe here an assay for assessing the relative cell permeability of synthetic molecules in the context of steroid fusions that is capable of high throughput and can be used in any transfectable cell line.

subject areas

  • Binding, Competitive
  • Cell Membrane Permeability
  • Combinatorial Chemistry Techniques
  • Drug Design
  • HeLa Cells
  • Humans
  • Peptide Library
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1087-0156

Digital Object Identifier (DOI)

  • 10.1038/nbt1099

PubMed ID

  • 15908941
scroll to property group menus

Additional Document Info

start page

  • 746

end page

  • 751

volume

  • 23

issue

  • 6

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support