related to degree

• Calarese, Daniel A, Ph.D. in Macromolecular and Cellular Structure and Chemistry, Scripps Research 2001 - 2006
• Huang, Cheng-Yuan, Ph.D. in Chemistry, Scripps Research 2000 - 2005
• Bryan, Marian C, Ph.D. in Chemistry, Scripps Research 1999 - 2004

authors

• Bryan, Marian C
• Fazio, F.
• Lee, H. K.
• Huang, Cheng-Yuan
• Chang, A.
• Best, M. D.
• Calarese, Daniel A
• Blixt, C.
• Paulson, James
• Burton, Dennis
• Wilson, Ian
• Wong, Chi-Huey

publication date

• July 2004

journal

• Journal of the American Chemical Society  Journal

abstract

• A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 muM).

subject areas

• Carbohydrate Sequence
• Combinatorial Chemistry Techniques
• Dithiothreitol
• Molecular Sequence Data
• Molecular Structure
• Oligosaccharides
• Spectrometry, Mass, Electrospray Ionization
• Triazoles

International Standard Serial Number (ISSN)

• 0002-7863

Digital Object Identifier (DOI)

• 10.1021/ja048433f

PubMed ID

• 15250702

start page

• 8640

end page

• 8641

volume

• 126

issue

• 28