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Evidence for kappa- and mu-opioid receptor expression in C6 glioma cells

Academic Article
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Overview

authors

  • Bohn, Laura
  • Belcheva, M. M.
  • Coscia, C. J.

publication date

  • 1998

journal

  • Journal of Neurochemistry  Journal

abstract

  • The astrocytoma cell line rat C6 glioma has been used as a model system to study the mechanism of various opioid actions. Nevertheless, the type of opioid receptor(s) involved has not been established. Here we demonstrate the presence of high-affinity U69,593, endomorphin-1, morphine, and beta-endorphin binding in desipramine (DMI)-treated C6 cell membranes by performing homologous and heterologous binding assays with [3H]U69,593, [3H]morphine, or 125I-beta-endorphin. Naive C6 cell membranes displayed U69,593 but neither endomorphin-1, morphine, nor beta-endorphin binding. Cross-linking of 125I-beta-endorphin to C6 membranes gave labeled bands characteristic of opioid receptors. Moreover, RT-PCR analysis of opioid receptor expression in control and DMI-treated C6 cells indicate that both kappa- and mu-opioid receptors are expressed. There does not appear to be a significant difference in the level of mu nor kappa receptor expression in naive versus C6 cells treated with DMI over a 20-h period. Collectively, the data indicate that kappa- and mu-opioid receptors are present in C6 glioma cells.

subject areas

  • Analgesics
  • Analgesics, Opioid
  • Animals
  • Benzeneacetamides
  • Binding, Competitive
  • Desipramine
  • Glioma
  • Morphine
  • Polymerase Chain Reaction
  • Pyrrolidines
  • Rats
  • Receptors, Opioid, kappa
  • Receptors, Opioid, mu
  • Transcription, Genetic
  • Tumor Cells, Cultured
  • beta-Endorphin
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Research

keywords

  • C6 glioma
  • PCR
  • U69,593
  • beta-endorphin
  • desipramine
  • morphine
  • opioid receptor
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Identity

PubMed Central ID

  • PMC2571951

International Standard Serial Number (ISSN)

  • 0022-3042

PubMed ID

  • 9572265
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Additional Document Info

start page

  • 1819

end page

  • 1825

volume

  • 70

issue

  • 5

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