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Bacillus subtilis muts mutl operon: Identification, nucleotide sequence and mutagenesis

Academic Article
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Overview

authors

  • Ginetti, F.
  • Perego, Marta
  • Albertini, A. M.
  • Galizzi, A.

publication date

  • August 1996

journal

  • Microbiology-SGM  Journal

abstract

  • The Bacillus subtilis mutS and mutL genes, involved in the DNA mismatch repair system, have been cloned and characterized. From sequence analysis the two genes appear to be organized in a single operon, located immediately downstream of the cotE gene (approximately 150 degrees on the genetic map). The deduced MutS protein is 49% identical to HexA and MutL is 46% identical to HexB of Streptococcus pneumoniae. Deletion of both mutS and mutL resulted in an increase in the frequency of spontaneous mutations and abolished the marker effect observed in transformation. The expression of the mut operon was studied with the use of a mutSL-lacZ transcriptional fusion. An increase in expression was observed during late exponential growth.

subject areas

  • Adenosine Triphosphatases
  • Amino Acid Sequence
  • Bacillus subtilis
  • Bacterial Proteins
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Primers
  • DNA Repair
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Genes, Bacterial
  • Genetic Vectors
  • Genotype
  • Molecular Sequence Data
  • MutS DNA Mismatch-Binding Protein
  • Operon
  • Phylogeny
  • Plasmids
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Homology, Amino Acid
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Research

keywords

  • Bacillus subtilis
  • DNA repair
  • mut operon
  • mutagenesis
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Identity

International Standard Serial Number (ISSN)

  • 1350-0872

PubMed ID

  • 8760914
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Additional Document Info

start page

  • 2021

end page

  • 2029

volume

  • 142

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