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Not more than 117 base-pairs of 5'-flanking sequence are required for inducible expression of a human ifn-alpha gene

Academic Article
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Overview

authors

  • Ragg, H.
  • Weissmann, Charles

publication date

  • 1983

journal

  • Nature  Journal

abstract

  • Interferon genes are usually only expressed after induction. In the accompanying paper we have shown that the accumulation of mRNA after viral induction is due to activation of transcription, rather than to reduction of turnover, and that the regulation of the alpha-interferon (IFN-alpha) gene is mediated by a segment of 5'-flanking region of not more than 700 base pairs (bp). To delineate the sequences required for induction, a set of 5' deletion mutants of the human IFN-alpha 1 gene was constructed and the expression of the truncated genes in mouse L cells was monitored after viral or mock infection. We report that not more than 117 bp of 5'-flanking sequence were required for induced expression of the gene. A purine-rich sequence of 42 bp located immediately downstream of position -117 is highly conserved in all known human alpha-interferon genes.

subject areas

  • Animals
  • Base Composition
  • Base Sequence
  • DNA Restriction Enzymes
  • Genes
  • Humans
  • Interferon Type I
  • L Cells (Cell Line)
  • Mice
  • Mutation
  • Plasmids
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/303439a0

PubMed ID

  • 6304525
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Additional Document Info

start page

  • 439

end page

  • 442

volume

  • 303

issue

  • 5916

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