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Anti-fibrillarin autoantibodies in mercury treated mice

Academic Article
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Overview

authors

  • Hultman, P.
  • Enestrom, S.
  • Pollard, Kenneth Michael
  • Tan, Eng

publication date

  • December 1989

journal

  • Clinical and Experimental Immunology  Journal

abstract

  • Using indirect immunofluorescence (IF) with HEp-2 cells as a substrate serially bled SJL mice were found to gradually develop a high titre of anti-nucleolar antibodies (ANuA) after 3-5 weeks of s.c. injections of 1.6 mg HgCl2/kg body weight every third day. The ANuA showed a clumpy nucleolar pattern of localization and were composed of all IgG subclasses, but contained, in comparison with the antinuclear antibodies (ANA) in MRL-lpr/lpr mice, significantly lower titres of IgG2a and only traces of IgG3. Immunoblotting analysis using purified mouse liver nucleoli revealed that the sera with ANuA identified the same 34-kD nucleolar protein which was targeted by a human scleroderma serum containing autoantibodies monospecific for fibrillarin. In addition, a fraction of the mercury-treated SJL mice developed serum antibodies reacting with 10-15 and 60-70 kD nucleolar proteins in immunoblotting. The presence of serum autoantibodies reacting with the 10-15 kD proteins correlated with significantly increased titres of anti-histone antibodies of the IgG class in ELISA. Some mercury-treated SJL mice also developed a significantly increased titre of anti-histone antibodies of the IgM class. B10.S mice treated with mercuric chloride consistently developed ANuA, which also targeted a 34-kD nucleolar protein. Since anti-fibrillarin antibodies are specific markers of scleroderma, the present animal model may be valuable for studies of the immunological aberrations which are likely to induce this autoimmune response.

subject areas

  • Animals
  • Antibodies, Antinuclear
  • Chromosomal Proteins, Non-Histone
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Immunoblotting
  • Immunoglobulin G
  • Mercuric Chloride
  • Mice
  • Scleroderma, Systemic
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Identity

PubMed Central ID

  • PMC1534831

International Standard Serial Number (ISSN)

  • 0009-9104

PubMed ID

  • 2612058
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Additional Document Info

start page

  • 470

end page

  • 477

volume

  • 78

issue

  • 3

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