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Novel lcmv-specific h-2k restricted ctl clones recognize internal viral gene-products and cause cns disease

Academic Article
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Overview

authors

  • Lewicki, H.
  • McKee, T. A.
  • Tishon, A.
  • Salvato, M.
  • Whitton, J. Lindsay
  • Oldstone, Michael

publication date

  • November 1992

journal

  • Journal of Neuroimmunology  Journal

abstract

  • H-2k (C3H/Hej) cytotoxic T lymphocytes (CTL) specific for lymphocytic choriomeningitis virus (LCMV) were cloned. Three clones recognizing internal viral antigens were studied. One such CTL clone recognized neither the glycoprotein nor nucleoprotein encoded by the viral short RNA segment, but reacted with a protein encoded by the long RNA segment, either the viral polymerase, or the Z protein. This one clone, in addition to primary CTL harvested from immunized C3H mice, failed to lyse target cells expressing the Z protein, suggesting recognition was to the viral polymerase. Two other clones recognized the viral nucleoprotein, amino acids 93-100, as determined by protein deletion and peptide mapping studies. When introduced directly into the central nervous systems of LCMV-infected histocompatible mice, all clones were active in vivo and capable of causing immunopathologically mediated death.

subject areas

  • Animals
  • Antigens, Viral
  • Base Sequence
  • Carrier Proteins
  • Central Nervous System Diseases
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Epitopes
  • Fatty Acid-Binding Proteins
  • H-2 Antigens
  • Immunity, Cellular
  • Immunotherapy, Adoptive
  • Lymphocytic choriomeningitis virus
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Peptide Mapping
  • T-Lymphocytes, Cytotoxic
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Identity

International Standard Serial Number (ISSN)

  • 0165-5728

Digital Object Identifier (DOI)

  • 10.1016/0165-5728(92)90190-v

PubMed ID

  • 1281166
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Additional Document Info

start page

  • 15

end page

  • 20

volume

  • 41

issue

  • 1

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