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DNA detection and signal amplification via an engineered allosteric enzyme

Academic Article
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Overview

related to degree

  • Thayer, Desiree, Ph.D. in Chemistry, Scripps Research 2001 - 2006
  • Saghatelian, Alan, Ph.D. in Chemistry, Scripps Research 1997 - 2002

authors

  • Saghatelian, Alan
  • Guckian, K. M.
  • Thayer, Desiree
  • Ghadiri, M. Reza

publication date

  • 2003

journal

  • Journal of the American Chemical Society  Journal

abstract

  • Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions.

subject areas

  • Bacillus cereus
  • DNA
  • DNA, Single-Stranded
  • Kinetics
  • Metalloendopeptidases
  • Oligopeptides
  • Protease Inhibitors
  • Signal Processing, Computer-Assisted
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Identity

PubMed Central ID

  • PMC2453066

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja027885u

PubMed ID

  • 12517141
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Additional Document Info

start page

  • 344

end page

  • 345

volume

  • 125

issue

  • 2

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