related to degree

• Thayer, Desiree, Ph.D. in Chemistry, Scripps Research 2001 - 2006
• Saghatelian, Alan, Ph.D. in Chemistry, Scripps Research 1997 - 2002

authors

• Saghatelian, Alan
• Guckian, K. M.
• Thayer, Desiree
• Ghadiri, M. Reza

publication date

• January 2003

journal

• Journal of the American Chemical Society  Journal

abstract

• Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions.

subject areas

• Bacillus cereus
• DNA
• DNA, Single-Stranded
• Kinetics
• Metalloendopeptidases
• Oligopeptides
• Protease Inhibitors
• Signal Processing, Computer-Assisted

PubMed Central ID

• PMC2453066

International Standard Serial Number (ISSN)

• 0002-7863

Digital Object Identifier (DOI)

• 10.1021/ja027885u

PubMed ID

• 12517141

start page

• 344

end page

• 345

volume

• 125

issue

• 2