Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Phosphorylation of RhoGDI by Src regulates Rho GTPase binding and cytosol-membrane cycling

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • DerMardirossian, Celine
  • Rocklin, G.
  • Seo, J. Y.
  • Bokoch, G. M.

publication date

  • 2006

journal

  • Molecular Biology of the Cell  Journal

abstract

  • Rho GTPases (Rac, Rho, and Cdc42) play important roles in regulating cell function through their ability to coordinate the actin cytoskeleton, modulate the formation of signaling reactive oxidant species, and control gene transcription. Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from RhoGDI complexes, followed by their reuptake after membrane cycling. We show here that Src kinase binds and phosphorylates RhoGDI both in vitro and in vivo at Tyr156. Analysis of Rho GTPase-RhoGDI complexes using in vitro assays of complexation and in vivo by coimmunoprecipitation analysis indicates that Src-mediated phosphorylation of Tyr156 causes a dramatic decrease in the ability of RhoGDI to form a complex with RhoA, Rac1, or Cdc42. Phosphomimetic mutation of Tyr156-->Glu results in the constitutive association of RhoGDI(Y156E) with the plasma membrane and/or associated cortical actin. Substantial cortical localization of tyrosine-phosphorylated RhoGDI is also observed in fibroblasts expressing active Src, where it is most evident in podosomes and regions of membrane ruffling. Expression of membrane-localized RhoGDI(Y156E) mutant is associated with enhanced cell spreading and membrane ruffling. These results suggest that Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane-cycling and activity.

subject areas

  • Animals
  • Cell Line, Transformed
  • Cell Membrane Structures
  • Cytosol
  • Fibroblasts
  • Guanine Nucleotide Dissociation Inhibitors
  • HeLa Cells
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Phosphorylation
  • Phosphotyrosine
  • Protein Binding
  • Protein Transport
  • rho GTP-Binding Proteins
  • rho Guanine Nucleotide Dissociation Inhibitor alpha
  • rho-Specific Guanine Nucleotide Dissociation Inhibitors
  • src-Family Kinases
scroll to property group menus

Identity

PubMed Central ID

  • PMC1635405

International Standard Serial Number (ISSN)

  • 1059-1524

Digital Object Identifier (DOI)

  • 10.1091/mbc.E06-06-0533

PubMed ID

  • 16943322
scroll to property group menus

Additional Document Info

start page

  • 4760

end page

  • 4768

volume

  • 17

issue

  • 11

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support