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Clonal cell lines produced by infection of neocortical neuroblasts using multiple oncogenes transduced by retroviruses

Academic Article
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Overview

authors

  • Chun, Jerold
  • Jaenisch, R.

publication date

  • April 1996

journal

  • Molecular and Cellular Neuroscience  Journal

abstract

  • Toward understanding the early molecular development of neocortical neurons, we report the generation of two clonal murine cell lines derived by sequential oncogenic retroviral infection of neocortical neuronal precursors. The resulting cell lines stably express the telencephalon-specific gene BF-1 and a gene enriched in the neocortical ventricular zone, vzg-1. They also express early neuronal but not glial markers, possess neuron-like processes without chemical synapses by ultrastructure, and appear pyramidal or bipolar with processes resembling apical dendrites, bifurcating and beaded axons, or growth cones. Immortalization appears to have occurred through the expression of oncogene combinations SV40 large T with vras or SV40 large T with both vsrc and vmyc. These cell lines may represent developing neocortical neuroblasts blocked from complete differentiation, and they should be useful in the isolation and analysis of genes involved in early neocortical development.

subject areas

  • Animals
  • Biomarkers
  • Blotting, Northern
  • Blotting, Western
  • Cell Size
  • Cell Transformation, Viral
  • Cells, Cultured
  • Cerebral Cortex
  • Clone Cells
  • DNA-Binding Proteins
  • Female
  • Forkhead Transcription Factors
  • Genes, Reporter
  • Immunohistochemistry
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron
  • Nerve Tissue Proteins
  • Pregnancy
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Lysophosphatidic Acid
  • Retroviridae
  • Transduction, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 1044-7431

Digital Object Identifier (DOI)

  • 10.1006/mcne.1996.0023

PubMed ID

  • 8793865
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Additional Document Info

start page

  • 304

end page

  • 321

volume

  • 7

issue

  • 4

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