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Current status of available standards for quality improvement of assays for detection of autoantibodies to nuclear and intracellular antigens

Academic Article
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Overview

authors

  • Nakamura, R. M.
  • Bylund, D. J.
  • Tan, Eng

publication date

  • 1994

journal

  • Journal of Clinical Laboratory Analysis  Journal

abstract

  • Considerable progress has been made in the improvement of clinical assays for the detection of autoantibodies to nuclear and intracellular antigens with the use of available World Health Organization (WHO) and Arthritis Foundation/Centers for Disease Control (AF/CDC) standards. The ultimate goal of standardization is for various clinical laboratory test results to be interchangeable and for an exchange of data to be done with confidence. This report discusses the available standards. In addition, significant technical problems and variations in methodologies for the detection of autoantibodies to intracellular antigens noted during a 4-year study by a European Consensus Study Group are detailed. Currently, there is a need for a future generation of reference preparations and standards that will show specific antibody reactivity on sensitive enzymes and immunoblotting assays. Standardization efforts should be done to characterize specific nuclear and cellular antigen preparations that may be of natural or of recombinant technology origin.

subject areas

  • Antibodies, Antinuclear
  • Autoantibodies
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Immunodiffusion
  • Immunologic Tests
  • Microscopy, Fluorescence
  • Reference Standards
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Research

keywords

  • AUTOANTIBODY ASSAYS
  • NUCLEAR AND INTRACELLULAR ANTIGENS
  • STANDARDS
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Identity

International Standard Serial Number (ISSN)

  • 0887-8013

Digital Object Identifier (DOI)

  • 10.1002/jcla.1860080605

PubMed ID

  • 7869174
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Additional Document Info

start page

  • 360

end page

  • 368

volume

  • 8

issue

  • 6

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