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Modulation of neutrophil influx in glomerulonephritis in the rat with antimacrophage inflammatory protein-2 (mip-2) antibody

Academic Article
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Overview

authors

  • Feng, L. L.
  • Xia, Y. Y.
  • Yoshimura, T.
  • Wilson, Curtis

publication date

  • March 1995

journal

  • Journal of Clinical Investigation  Journal

abstract

  • The role of the chemokine, macrophage inflammatory protein-2 (MIP-2), during anti-glomerular basement membrane (GBM) antibody (Ab) glomerulonephritis (GN) was studied. Rat MIP-2 cDNA had been cloned previously. Recombinant rat MIP-2 (rMIP-2) from Escherichia coli exhibited neutrophil chemotactic activity and produced neutrophil influx when injected into the rat bladder wall. By using a riboprobe derived from the cDNA and an anti-rMIP-2 polyclonal Ab, MIP-2 was found to be induced in glomeruli with anti-GBM Ab GN as mRNA by 30 min and protein by 4 h, with both disappearing by 24 h. The expression of MIP-2 correlated with glomerular neutrophil influx. A single dose of the anti-MIP-2 Ab 30 min before anti-GBM Ab was effective in reducing neutrophil influx (40% at 4 h, P < 0.01) and periodic acid-Schiff deposits containing fibrin (54% at 24 h, P < 0.01). The anti-rMIP-2 Ab had no effect on anti-GBM Ab binding (paired-label isotope study). Functional improvement in the glomerular damage was evidenced by a reduction of abnormal proteinuria (P < 0.05). These results suggest that MIP-2 is a major neutrophil chemoattractant contributing to influx of neutrophils in Ab-induced glomerular inflammation in the rat.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antibody Specificity
  • Base Sequence
  • Chemokine CXCL2
  • Chemotaxis, Leukocyte
  • Cloning, Molecular
  • Cytokines
  • Dose-Response Relationship, Drug
  • Escherichia coli
  • Gene Expression Regulation
  • Glomerulonephritis
  • In Situ Hybridization
  • Intercellular Adhesion Molecule-1
  • Interleukin-1
  • Kidney Glomerulus
  • Lung
  • Molecular Sequence Data
  • Monokines
  • Neutrophils
  • Rats
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Urinary Bladder
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Research

keywords

  • ANTIGLOMERULAR BASEMENT MEMBRANE GLOMERULONEPHRITIS
  • ANTIMACROPHAGE INFLAMMATORY PROTEIN-2 ANTIBODY
  • CHEMOKINE
  • MACROPHAGE INFLAMMATORY PROTEIN-2
  • NEUTROPHILS
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Identity

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci117745

PubMed ID

  • 7883948
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Additional Document Info

start page

  • 1009

end page

  • 1017

volume

  • 95

issue

  • 3

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