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Contribution of receptor editing to the antibody repertoire

Academic Article
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Overview

authors

  • Casellas, R.
  • Shih, T. A. Y.
  • Kleinewietfeld, M.
  • Rakonjac, J.
  • Nemazee, David
  • Rajewsky, K.
  • Nussenzweig, M. C.

publication date

  • February 2001

journal

  • Science  Journal

abstract

  • Receptor editing, clonal deletion, and anergy are the mechanisms by which B cells maintain tolerance to self antigens. To determine the extent to which receptor editing shapes the normal antibody repertoire, we generated an immunoglobulin kappa polymorphism that facilitates the detection of editing of immunoglobulin light chains in vivo. We found that B cells are targeted for editing during a 2-hour delay in development at the pre-BII cell stage, and that about 25% of all antibody molecules are produced by gene replacement. These results suggest that receptor editing represents a major force in shaping the antibody repertoire.

subject areas

  • Animals
  • Antibody Affinity
  • B-Lymphocytes
  • Binding Sites, Antibody
  • DNA-Binding Proteins
  • Gene Rearrangement, B-Lymphocyte, Light Chain
  • Genes, Immunoglobulin
  • Hematopoietic Stem Cells
  • Humans
  • Immunoglobulin Constant Regions
  • Immunoglobulin Heavy Chains
  • Immunoglobulin kappa-Chains
  • Mice
  • Mice, Transgenic
  • Models, Immunological
  • Nuclear Proteins
  • Receptors, Antigen, B-Cell
  • Recombination, Genetic
  • Self Tolerance
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1056600

PubMed ID

  • 11222858
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Additional Document Info

start page

  • 1541

end page

  • 1544

volume

  • 291

issue

  • 5508

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