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Rapid, simple and sensitive method for demonstration of central catecholamine-containing neurons and axons by glyoxylic acid-induced fluorescence .2. Detailed description of methodology

Academic Article
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Overview

authors

  • Bloom, Floyd
  • Battenberg, E. L. F.

publication date

  • 1976

journal

  • Journal of Histochemistry & Cytochemistry  Journal

abstract

  • The glyoxylic acid-induced fluorescence method for localization of brain catecholamine neurons has been modified. Fluorescence is developed rapidly in cryostat sections of brains fixed by perfusion with 0.5% depolymerized paraformaldehyde and 2.0% glyoxylic acid. Since neither freeze drying nor vibratome sectioning is required, total processing time can be less than 1 hr. Both perikarya and fine varicose axons of norepinephrine- and dopamine-containing neurons can be seen throughout the neuroaxis. The modified technique retains good cytologic integrity and may provide a useful alternative for methods combining histochemical approaches.

subject areas

  • Animals
  • Axons
  • Binding Sites
  • Brain Chemistry
  • Catecholamines
  • Female
  • Glyoxylates
  • Male
  • Microscopy, Fluorescence
  • Neurons
  • Norepinephrine
  • Rats
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Identity

International Standard Serial Number (ISSN)

  • 0022-1554

PubMed ID

  • 1270793
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Additional Document Info

start page

  • 561

end page

  • 571

volume

  • 24

issue

  • 4

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