Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Deletions within the 5 ' utr of coxsackievirus b3: Consequences for virus translation and replication

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Hunziker, I. P.
  • Cornell, C. T.
  • Whitton, J. Lindsay

publication date

  • March 2007

journal

  • Virology  Journal

abstract

  • Key features of an ideal RNA-based vaccine against coxsackievirus B3 (CVB3) are (i) limited genome replication/virus production (to minimize vaccine-related pathology) and (ii) abundant virus protein synthesis (to maximize immunogenicity). These attributes may apply to CVB3 RNAs lacking up to 250 nucleotides (nt) from their 5' terminus; these RNAs do not give rise to infectious progeny, but they have been reported to retain the entire CVB3 IRES (mapped to nt approximately 432-639) and to produce large quantities of viral protein in transfected cells. Here, we constructed five 5' RNA deletion variants that, to our surprise, failed to protect against CVB3 challenge. We investigated the reasons for this failure and conclude that (i) a 5' terminal deletion as short as 32 nt abolishes CVB3 RNA replication in transfected cells; (ii) this deleted RNA, and others with longer deletions, do not direct abundant protein synthesis in transfected cells, probably as a consequence of their replicative incapacity; and (iii) the CVB3 IRES is substantially larger than previously thought, and its 5' boundary lies between residues 76 and 125, very closely approximating that of the poliovirus IRES.

subject areas

  • 5' Untranslated Regions
  • Animals
  • Coxsackievirus Infections
  • Down-Regulation
  • Enterovirus
  • Gene Deletion
  • HeLa Cells
  • Humans
  • Injections, Intramuscular
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Protein Biosynthesis
  • Vaccination
  • Vaccines, Synthetic
  • Viral Vaccines
  • Virus Replication
scroll to property group menus

Research

keywords

  • RNA vaccine
  • TRES
  • coxsackievirus
  • picomavirus
scroll to property group menus

Identity

PubMed Central ID

  • PMC2190293

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2006.09.041

PubMed ID

  • 17084431
scroll to property group menus

Additional Document Info

start page

  • 120

end page

  • 128

volume

  • 360

issue

  • 1

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support