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Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro

Academic Article
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Overview

authors

  • Peck, L. J.
  • Millstein, L.
  • Eversolecire, P.
  • Gottesfeld, Joel
  • Varshavsky, A.

publication date

  • October 1987

journal

  • Molecular and Cellular Biology  Journal

abstract

  • An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.

subject areas

  • Animals
  • Cloning, Molecular
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Deoxyribonuclease I
  • Gene Expression Regulation
  • Genes
  • Oocytes
  • Plasmids
  • RNA, Ribosomal
  • RNA, Ribosomal, 5S
  • Transcription Factors
  • Transcription, Genetic
  • Xenopus laevis
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Identity

PubMed Central ID

  • PMC368002

International Standard Serial Number (ISSN)

  • 0270-7306

PubMed ID

  • 3683391
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Additional Document Info

start page

  • 3503

end page

  • 3510

volume

  • 7

issue

  • 10

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