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Efficient peripheral clonal elimination of b lymphocytes in mrl/lpr mice bearing autoantibody transgenes

Academic Article
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Overview

authors

  • Kench, J. A.
  • Russell, D. M.
  • Nemazee, David

publication date

  • September 1998

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Peripheral B cell tolerance was studied in mice of the autoimmune-prone, Fas-deficient MRL/ lpr.H-2(d) genetic background by introducing a transgene that directs expression of membrane-bound H-2Kb antigen to liver and kidney (MT-Kb) and a second transgene encoding antibody reactive with this antigen (3-83mu delta, anti-Kk,b). Control immunoglobulin transgenic (Ig-Tg) MRL/lpr.H-2(d) mice lacking the Kb antigen had large numbers of splenic and lymph node B cells bearing the transgene-encoded specificity, whereas B cells of the double transgenic (Dbl-Tg) MRL/lpr.H-2(d) mice were deleted as efficiently as in Dbl-Tg mice of a nonautoimmune B10.D2 genetic background. In spite of the severely restricted peripheral B cell repertoire of the Ig-Tg MRL/lpr.H-2(d) mice, and notwithstanding deletion of the autospecific B cell population in the Dbl-Tg MRL/lpr.H-2(d) mice, both types of mice developed lymphoproliferation and exhibited elevated levels of IgG anti-chromatin autoantibodies. Interestingly, Dbl-Tg MRL/lpr.H-2(d) mice had a shorter lifespan than Ig-Tg MRL/lpr.H-2(d) mice, apparently as an indirect result of their relative B cell lymphopenia. These data suggest that in MRL/lpr mice peripheral B cell tolerance is not globally defective, but that certain B cells with receptors specific for nuclear antigens are regulated differently than are cells reactive to membrane autoantigens.

subject areas

  • Aging
  • Animals
  • Autoantibodies
  • B-Lymphocytes
  • Clonal Deletion
  • H-2 Antigens
  • Hyperplasia
  • Immune Sera
  • Lymph Nodes
  • Lymphoproliferative Disorders
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred MRL lpr
  • Mice, Transgenic
  • Transgenes
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Research

keywords

  • B lymphocyte
  • MRL/lpr
  • autoantibody
  • systemic lupus erythematosus
  • tolerance
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.188.5.909

PubMed ID

  • 9730892
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Additional Document Info

start page

  • 909

end page

  • 917

volume

  • 188

issue

  • 5

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