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Gp33-38, an early human T-cell activation antigen

Academic Article
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Overview

authors

  • Carrel, S.
  • Salvi, S.
  • Isler, P.
  • Rapin, C.
  • Hayoz, D.
  • Gallay, Philippe
  • Giuffre, L.

publication date

  • 1990

journal

  • Journal of Immunology  Journal

abstract

  • We describe an activation Ag Me14/D12 that appears early after T cell activation and is absent in resting T lymphocytes. Me14/D12 is a nondisulfide-linked heterodimeric structure containing two polypeptide chains of 33,000 and 38,000 Da. The expression of Me14/D12 on resting T lymphocytes can be induced by different activation stimuli such as the lectins PHA and Con A, the phorbol ester PMA, and anti-CD3 mAb. The induction of mRNA for Me14/D12 (gp33-38) in PHA-activated T lymphocytes precedes that of IL-2R gene transcripts by more than 20 h. Me14/D12 mRNA was detectable as early as 2 h after the onset of activation and mRNA for the IL-2R only after 24 h. The surface expression of Me14/D12 was detectable between 12 and 24 h after activation and was maximal between 24 and 48 h. Several T leukemia cell lines express the Me14/D12 Ag. On Me14/D12- cell lines, PMA and IFN-gamma induced surface expression of Me14/D12. Once Me14/D12 Ag were expressed on Jurkat cells after stimulation with either PMA or IFN-gamma, the binding of mAb Me14/D12 induced the production of significant amounts of IL-2 and of Ca2+ mobilization from internal stores. Comparative biochemical studies clearly demonstrate that Me14/D12 (gp33-38) is different from the CD69 molecular complex defined by mAb MLR3 and AIM.

subject areas

  • Antibodies, Monoclonal
  • Antigens, CD3
  • Antigens, Differentiation, T-Lymphocyte
  • Cell Line
  • Hematopoietic Stem Cells
  • Humans
  • Interferon-gamma
  • Isoelectric Point
  • Leukemia, T-Cell
  • Lymphocyte Activation
  • Membrane Glycoproteins
  • Molecular Weight
  • Phytohemagglutinins
  • Precipitin Tests
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes
  • Tetradecanoylphorbol Acetate
  • Time Factors
  • Tumor Cells, Cultured
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 2138192
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Additional Document Info

start page

  • 2053

end page

  • 2062

volume

  • 144

issue

  • 6

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