Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Recruitment of epidermal growth-factor and transferrin receptors into coated pits in-vitro - differing biochemical requirements

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Lamaze, C.
  • Baba, T.
  • Redelmeier, T. E.
  • Schmid, Sandra

publication date

  • July 1993

journal

  • Molecular Biology of the Cell  Journal

abstract

  • The biochemical requirements for epidermal growth factor (EGF) and transferrin receptor-mediated endocytosis were compared using perforated human A431 cells. Morphological studies showed that horseradish peroxidase (HRP)-conjugated EGF and gold-labeled antitransferrin (Tfn) receptor antibodies were colocalized during endocytosis in vitro. The sequestration of both ligands into deeply invaginated coated pits required ATP hydrolysis and cytosolic factors and was inhibited by GTP gamma S, indicating mechanistic similarities. Importantly, several differences in the biochemical requirements for sequestration of EGF and Tfn were also detected. These included differing requirements for soluble AP (clathrin assembly protein) complexes, differing cytosolic requirements, and differing sensitivities to the tyrosine kinase inhibitor, genistein. The biochemical differences detected between EGF and Tfn sequestration most likely reflect specific requirements for the recruitment of EGF-receptors (R) into coated pits. This assay provides a novel means to identify the molecular bases for these biochemical distinctions and to elucidate the mechanisms involved in ligand-induced recruitment of EGF-R into coated pits.

subject areas

  • Adenosine Triphosphate
  • Adenylyl Imidodiphosphate
  • Carcinoma, Squamous Cell
  • Cell Membrane Permeability
  • Coated Pits, Cell-Membrane
  • Cytosol
  • Egtazic Acid
  • Endocytosis
  • Enzyme-Linked Immunosorbent Assay
  • Epidermal Growth Factor
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Humans
  • Kinetics
  • Ligands
  • Microscopy, Immunoelectron
  • Receptor, Epidermal Growth Factor
  • Receptors, Transferrin
  • Tumor Cells, Cultured
scroll to property group menus

Identity

PubMed Central ID

  • PMC300981

International Standard Serial Number (ISSN)

  • 1059-1524

PubMed ID

  • 8400457
scroll to property group menus

Additional Document Info

start page

  • 715

end page

  • 727

volume

  • 4

issue

  • 7

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support