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Identification of lipoxin-a4 and its relationship to the sulfidopeptide leukotriene-c4, leukotriene-d4, and leukotriene-e4 in the bronchoalveolar lavage fluids obtained from patients with selected pulmonary-diseases

Academic Article
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Overview

authors

  • Lee, T. H.
  • Crea, A. E. G.
  • Gant, V.
  • Spur, B. W.
  • Marron, B. E.
  • Nicolaou, K.C.
  • Reardon, E.
  • Brezinski, M.
  • Serhan, C. N.

publication date

  • June 1990

journal

  • American Review of Respiratory Disease  Journal

abstract

  • Lipoxins are biologically active trihydroxytetraene containing products derived from arachidonic acid that are formed by interactions between lipoxygenases. Although the lipoxins have been generated from mixed cell suspensions in vitro, it has not been established whether these products are synthesized in vivo. We have performed bronchoalveolar lavage (BAL) in 12 patients with lung disease (sarcoid, six; pneumonia, two; asthma, two; carcinoma, one; alveolitis of unknown cause, one) and in six normal control subjects. The BAL fluid was analyzed for lipoxin A4 (LXA4) using gas chromatography mass spectrometry with selective ion monitoring, and the levels of the sulfidopeptide leukotrienes were determined using reverse-phase high-performance liquid chromatography and radioimmunoassay. LXA4 was detected in BAL fluid from nine of the 12 patients studied. The levels of LXA4 ranged from 0.4 to 2.8 ng/ml. LXA4 was not detected in any of the six normal subjects. Sulfidopeptide leukotrienes were detected in all the BAL samples, ranging from 0.04 to 0.7 ng/ml, and there was no significant difference between the patients and the normal subjects. In patients with detectable LXA4 in BAL fluid, the ratio of the concentrations of LXA4 to those of the sulfidopeptide leukotrienes ranged from 1.9 to 62 (mean, 19.0). This is the first demonstration of the presence of LXA4 in disease.

subject areas

  • Adult
  • Bronchoalveolar Lavage Fluid
  • Chromatography, High Pressure Liquid
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Hydroxyeicosatetraenoic Acids
  • Leukotriene B4
  • Leukotriene E4
  • Lipoxins
  • Lung Diseases
  • Male
  • Middle Aged
  • Radioimmunoassay
  • SRS-A
  • Sarcoidosis
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Identity

International Standard Serial Number (ISSN)

  • 0003-0805

PubMed ID

  • 2161628
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Additional Document Info

start page

  • 1453

end page

  • 1458

volume

  • 141

issue

  • 6

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