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Quantitative-analysis of transforming growth-factor-beta-1 messenger-rna in the liver of patients with chronic hepatitis-c - absence of correlation between high-levels and severity of disease

Academic Article
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Overview

authors

  • Roulot, D.
  • Durand, H.
  • Coste, T.
  • Rautureau, J.
  • Strosberg, Donny
  • Benarous, R.
  • Marullo, S.

publication date

  • February 1995

journal

  • Hepatology  Journal

abstract

  • Transforming growth factor beta 1 (TGF beta 1) is a cytokine involved in liver fibrogenesis. Previous semiquantitative studies of patients with chronic viral hepatitis showed that liver TGF beta 1 messenger RNA (mRNA) was increased, compared with normal controls and with patients with chronic hepatitis C virus (HCV) infection who responded favorably to interferon alfa (IFN alpha) treatment. To evaluate its potential prognostic significance, we measured liver TGF beta 1 mRNA, using a new competitive reverse gene amplification assay, in a total of 35 patients with chronic HCV. This technique was reproducible and sensitive; we could measure as few as 5,000 molecules of TGF beta 1 mRNA per microgram of total liver RNA. In patients with chronic HCV, the mean level of TGF beta 1 mRNA was 200-fold higher than in controls. However, no correlation could be found between TGF beta 1 mRNA and either the biological (serum amino-terminal peptide of type III procollagen) and histological (Knodell scores) indices of liver fibrosis or a favorable response to IFN alpha therapy. In 9 patients, second liver specimens were obtained after treatment; in most cases, TGF beta 1 mRNA levels and hepatic histological findings varied in parallel. These data are consistent with the hypothesis that TGF beta 1 plays a role in stimulating liver fibrogenesis during chronic HCV, despite the lack of prognostic value of TGF beta 1 mRNA levels measured before treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

subject areas

  • Adult
  • Aged
  • Base Sequence
  • Chronic Disease
  • Female
  • Hepatitis C
  • Humans
  • Liver
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Procollagen
  • RNA, Messenger
  • Severity of Illness Index
  • Transforming Growth Factor beta
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Identity

International Standard Serial Number (ISSN)

  • 0270-9139

Digital Object Identifier (DOI)

  • 10.1016/0270-9139(95)90084-5

PubMed ID

  • 7843697
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Additional Document Info

start page

  • 298

end page

  • 304

volume

  • 21

issue

  • 2

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