Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

A string-of-beads vaccine, comprising linked minigenes, confers protection from lethal-dose virus challenge

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Whitton, J. Lindsay
  • Sheng, N.
  • Oldstone, Michael
  • McKee, T. A.

publication date

  • January 1993

journal

  • Journal of Virology  Journal

abstract

  • We have previously demonstrated that induction of antiviral cytotoxic T lymphocytes (CTL), in the absence of antiviral antibodies, can confer protection against a lethal-dose virus challenge. Here we extend those findings as follows. First, three discrete viral CTL epitopes expressed from minigenes encoding peptides as short as 12 amino acids can be recognized when expressed from recombinant vaccinia virus; second, concentrating on two of the three epitopes, we show that these vaccinia virus recombinants can confer protection in a major histocompatibility complex (MHC)-restricted manner; third, the minigenes can be fused to generate a "string of beads," and the close proximity of the two epitopes within one oligopeptide does not disrupt recognition of either epitope; fourth, this string-of-beads vaccine, in contrast to the single epitope vaccines, can protect on both MHC backgrounds; and, fifth, CTL to different epitopes may act synergistically, as protection is improved when the vaccine contains more than one CTL epitope for a given MHC background.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytotoxicity, Immunologic
  • Epitopes
  • Genes, Viral
  • Genetic Vectors
  • Lymphocytic Choriomeningitis
  • Major Histocompatibility Complex
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Peptide Fragments
  • Recombinant Proteins
  • T-Lymphocytes, Cytotoxic
  • Vaccines, Synthetic
  • Vaccinia virus
  • Viral Proteins
scroll to property group menus

Identity

PubMed Central ID

  • PMC237369

International Standard Serial Number (ISSN)

  • 0022-538X

PubMed ID

  • 7677954
scroll to property group menus

Additional Document Info

start page

  • 348

end page

  • 352

volume

  • 67

issue

  • 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support