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Monoclonal antibody-directed determination of cytochrome P-450 types expressed in a human lymphoblastoid cell line

Academic Article
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Overview

authors

  • Friedman, F. K.
  • Robinson, R. C.
  • Song, B. J.
  • Park, S. S.
  • Crespi, C. L.
  • Marletta, Michael
  • Gelboin, H. V.

publication date

  • 1985

journal

  • Molecular Pharmacology  Journal

abstract

  • Cytochrome P-450-dependent aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin O-deethylase activities of a cloned line of human lymphoblastoid AHH-1 cells are inhibited by a monoclonal antibody (MAb 1-7-1) prepared to a 3-methylcholanthrene-induced rat liver cytochrome P-450. The monoclonal antibody inhibition determined that a single MAb 1-7-1-sensitive type of cytochrome P-450 is responsible for all of AHH expression in both the basal and benz[a]anthracene-induced cells. Partial inhibition by the MAb 1-7-1, however, indicates that at least two forms of cytochrome P-450 catalyze 7-ethoxycoumarin O-deethylase in both the basal and the induced cells, one form of which is identical to the MAb-sensitive cytochrome P-450 responsible for all of the AHH. Thus, a single cloned cell line is capable of expressing two classes of cytochromes P-450, and the observed multiplicity of cytochrome P-450 in animal tissues does not necessarily depend on cell heterogeneity. A sensitive MAb 1-7-1-based radioimmunoassay also directly demonstrates the presence in these cells of a MAb 1-7-1-specific type of cytochrome P-450 as well as its elevation in the induced cells. These MAb-based methods thus can determine the contribution of specific MAb-defined types of cytochromes P-450 to the cellular metabolism of specific xenobiotics.

subject areas

  • 7-Alkoxycoumarin O-Dealkylase
  • Animals
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Aryl Hydrocarbon Hydroxylases
  • Benz(a)Anthracenes
  • Cell Line
  • Clone Cells
  • Cytochrome P-450 Enzyme System
  • Humans
  • Kinetics
  • Lymphocytes
  • Methylcholanthrene
  • Microsomes, Liver
  • Oxygenases
  • Radioimmunoassay
  • Rats
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Identity

International Standard Serial Number (ISSN)

  • 0026-895X

PubMed ID

  • 3873598
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Additional Document Info

start page

  • 652

end page

  • 655

volume

  • 27

issue

  • 6

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