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Interleukin 1 expression is inducible by nerve growth factor in PC12 pheochromocytoma cells

Academic Article
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Overview

authors

  • Alheim, K.
  • Andersson, C.
  • Tingsborg, S.
  • Ziolkowska, M.
  • Schultzberg, M.
  • Bartfai, Tamas

publication date

  • October 1991

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Expression of the cytokine interleukin 1 alpha (IL-1 alpha) was demonstrated in the rat PC12 pheochromocytoma cell line by (i) immunohistochemistry using rabbit polyclonal antisera raised against the recombinant murine IL-1 alpha, (ii) an ELISA, and (iii) a specific cell conversion bioassay based on the use of LBRM33-1A5 cells. IL-1 alpha mRNA was demonstrated in the PC12 cells, by PCR amplification. Constitutive expression of IL-1 alpha in PC12 cells was demonstrated in all experiments, although the cellular levels of IL-1 alpha-like immunoreactivity varied. The expression of IL-1 alpha, as studied at the mRNA level, was inducible by mouse nerve growth factor (7S NGF), and the gene product level was inducible in a dose- and time-dependent fashion by 7S NGF. The maximum induction corresponds to a 600% increase in IL-1 alpha-like immunoreactivity above the expression level found in noninduced cells and occurred after a 3-day incubation of the cells with NGF at 0.75 micrograms/ml of culture medium. The significance of the ability of NGF to induce IL-1 expression lies in the fact that IL-1 itself also acts as a growth factor that promotes glial proliferation and, even more importantly, IL-1 itself induces the expression of NGF at peripheral nerve injury [Lindholm, D., Heumann, R., Meyer, M. & Thoenen, H. (1987) Nature (London) 330, 658-659].

subject areas

  • Animals
  • Antibodies
  • Base Sequence
  • Biological Assay
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Immunohistochemistry
  • Interleukin-1
  • Molecular Sequence Data
  • Nerve Growth Factors
  • Oligodeoxyribonucleotides
  • PC12 Cells
  • Polymerase Chain Reaction
  • RNA, Neoplasm
  • Recombinant Proteins
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Identity

PubMed Central ID

  • PMC52702

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.20.9302

PubMed ID

  • 1924394
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Additional Document Info

start page

  • 9302

end page

  • 9306

volume

  • 88

issue

  • 20

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