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Generation and phenotypic analysis of protein S-deficient mice

Academic Article
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Overview

authors

  • Saller, F.
  • Brisset, A. C.
  • Tchaikovski, S. N.
  • Azevedo, M.
  • Chrast, R.
  • Fernandez, J. A.
  • Schapira, M.
  • Hackeng, T. M.
  • Griffin, John
  • Angelillo-Scherrer, A.

publication date

  • September 2009

journal

  • Blood  Journal

abstract

  • Protein S (PS) is an important natural anticoagulant with potentially multiple biologic functions. To investigate further the role of PS in vivo, we generated Pros(+/-) heterozygous mice. In the null (-) allele, the Pros exons 3 to 7 have been excised through conditional gene targeting. Pros(+/-) mice did not present any signs of spontaneous thrombosis and had reduced PS plasma levels and activated protein C cofactor activity in plasma coagulation and thrombin generation assays. Tissue factor pathway inhibitor cofactor activity of PS could not be demonstrated. Heterozygous Pros(+/-) mice exhibited a notable thrombotic phenotype in vivo when challenged in a tissue factor-induced thromboembolism model. No viable Pros(-/-) mice were obtained through mating of Pros(+/-) parents. Most E17.5 Pros(-/-) embryos were found dead with severe intracranial hemorrhages and most likely presented consumptive coagulopathy, as demonstrated by intravascular and interstitial fibrin deposition and an increased number of megakaryocytes in the liver, suggesting peripheral thrombocytopenia. A few E17.5 Pros(-/-) embryos had less severe phenotype, indicating that life-threatening manifestations might occur between E17.5 and the full term. Thus, similar to human phenotypes, mild heterozygous PS deficiency in mice was associated with a thrombotic phenotype, whereas total homozygous deficiency in PS was incompatible with life.

subject areas

  • Animals
  • Disease Models, Animal
  • Fetal Death
  • Heterozygote
  • Humans
  • Intracranial Hemorrhages
  • Lipoproteins
  • Liver
  • Megakaryocytes
  • Mice
  • Mice, Knockout
  • Protein C
  • Protein S
  • Protein S Deficiency
  • Thrombin
  • Thromboembolism
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Identity

PubMed Central ID

  • PMC2745849

International Standard Serial Number (ISSN)

  • 0006-4971

Digital Object Identifier (DOI)

  • 10.1182/blood-2009-03-209031

PubMed ID

  • 19567881
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Additional Document Info

start page

  • 2307

end page

  • 2314

volume

  • 114

issue

  • 11

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