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Mimicry of human histocompatibility HLA-B27 antigens by Klebsiella-pneumoniae

Academic Article
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Overview

authors

  • Ogasawara, M.
  • Kono, Dwight
  • Yu, D. T. Y.

publication date

  • 1986

journal

  • Infection and Immunity  Journal

abstract

  • Anti-HLA-B27 monoclonal antibody M2, which was relatively specific for human histocompatibility antigen HLA-B27, was used to test several bacteria, some of which could potentially induce chronic arthritis in HLA-B27-positive individuals. Using the Western blot procedure, we observed positive reactions with 80,000- and 60,000-dalton antigens with one strain of Klebsiella pneumoniae. Reactivity was not observed with five other monoclonal antibodies which were not reactive with HLA-B27 antigens, nor was reactivity observed with seven other gram-negative bacteria, irrespective of their arthritis-causing potential. To test the validity of our observation, the 80,000-dalton Klebsiella cross-reactive antigen was isolated and used to generate an immune guinea pig serum. We found that the reactivity of this guinea pig serum with Klebsiella envelopes in an enzyme-linked immunosorbent assay was adversely affected by absorption with HLA-B27-positive cells. Our results support the existence of mimicry between HLA-B27 antigens and bacteria.

subject areas

  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Cross Reactions
  • Cytotoxicity Tests, Immunologic
  • Detergents
  • Enzyme-Linked Immunosorbent Assay
  • HLA Antigens
  • HLA-B27 Antigen
  • Immunosorbent Techniques
  • Klebsiella pneumoniae
  • Molecular Weight
  • Solubility
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Identity

PubMed Central ID

  • PMC260984

International Standard Serial Number (ISSN)

  • 0019-9567

PubMed ID

  • 3512439
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Additional Document Info

start page

  • 901

end page

  • 908

volume

  • 51

issue

  • 3

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