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Secretion of a chimeric t-cell receptor immunoglobulin protein

Academic Article
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Overview

authors

  • Gascoigne, Nicholas
  • Goodnow, C. C.
  • Dudzik, K. I.
  • Oi, V. T.
  • Davis, M. M.

publication date

  • May 1987

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • To produce sufficient quantities of soluble T-cell receptor protein for detailed biochemical and biophysical analyses we have explored the use of immunoglobulin--T-cell receptor gene fusions. In this report we describe a chimeric gene construct containing a T-cell receptor alpha-chain variable (V) domain and the constant (C) region coding sequences of an immunoglobulin gamma 2a molecule. Cells transfected with the chimeric gene synthesize a stable protein product that expresses immunoglobulin and T-cell receptor antigenic determinants as well as protein A binding sites. We show that the determinant recognized by the anticlonotypic antibody A2B4.2 resides on the V alpha domain of the T-cell receptor. The chimeric protein associates with a normal lambda light chain to form an apparently normal tetrameric (H2L2, where H = heavy and L = light) immunoglobulin molecule that is secreted. Also of potential significance is the fact that a T-cell receptor V beta gene in the same construct is neither assembled nor secreted with the lambda light chain, and when expressed with a C kappa region it does not assemble with the chimeric V alpha C gamma 2a protein mentioned above. This indicates that not all T-cell receptor V regions are similar enough to immunoglobulin V regions for them to be completely interchangeable.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antibodies
  • Base Sequence
  • Cell Line
  • Chimera
  • Cloning, Molecular
  • Genes
  • Immunoglobulin Variable Region
  • Mice
  • Plasmacytoma
  • Promoter Regions, Genetic
  • Receptors, Antigen, T-Cell
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.9.2936

PubMed ID

  • 3472243
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Additional Document Info

start page

  • 2936

end page

  • 2940

volume

  • 84

issue

  • 9

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