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Direct association between the yeast rad51 and rad54 recombination proteins

Academic Article
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Overview

authors

  • Jiang, H.
  • Xie, Y. Q.
  • Houston, P.
  • Stemkehale, K.
  • Mortensen, U. H.
  • Rothstein, R.
  • Kodadek, Thomas

publication date

  • 1996

journal

  • Journal of Biological Chemistry  Journal

abstract

  • The RAD54 and RAD51 genes are involved in genetic recombination and double-strand break repair in the yeast Saccharomyces cerevisiae. The Rad51 protein is thought to be a yeast analogue of the Eschericia coli recA gene product and catalyzes strand exchange between homologous single- and double-stranded DNAs in vitro. RAD54 exhibits homologies to several known ATPases and is a member of the SWI2/MOT1 family. We show here that the Rad54 protein interacts with the Rad51 protein in vivo and in vitro and that the NH2-terminal 115 residues of the Rad54 protein are necessary for this interaction. Combined with previously reported results, these data imply that the Rad54 protein is part of a multiprotein yeast recombination complex.

subject areas

  • Binding Sites
  • Blotting, Western
  • DNA Helicases
  • DNA Repair Enzymes
  • DNA-Binding Proteins
  • Deoxyribonuclease BamHI
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes
  • Fungal Proteins
  • Rad51 Recombinase
  • Recombinant Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • beta-Galactosidase
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 8969173
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Additional Document Info

start page

  • 33181

end page

  • 33186

volume

  • 271

issue

  • 52

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