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RNA-catalyzed RNA ligation on an external RNA template

Academic Article
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Overview

related to degree

  • McGinness, Kathleen, Ph.D. in Chemistry, Scripps Research 1996 - 2001

authors

  • McGinness, Kathleen
  • Joyce, Gerald

publication date

  • 2002

journal

  • Chemistry & Biology  Journal

abstract

  • Variants of the hc ligase ribozyme, which catalyzes ligation of the 3' end of an RNA substrate to the 5' end of the ribozyme, were utilized to evolve a ribozyme that catalyzes ligation reactions on an external RNA template. The evolved ribozyme catalyzes the joining of an oligonucleotide 3'-hydroxyl to the 5'-triphosphate of an RNA hairpin molecule. The ribozyme can also utilize various substrate sequences, demonstrating a largely sequence-independent mechanism for substrate recognition. The ribozyme also carries out the ligation of two oligonucleotides that are bound at adjacent positions on a complementary template. Finally, it catalyzes addition of mononucleoside 5'-triphosphates onto the 3' end of an oligonucleotide primer in a template-dependent manner. The development of ribozymes that catalyze polymerase-type reactions contributes to the notion that an RNA world could have existed during the early history of life on Earth.

subject areas

  • Directed Molecular Evolution
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Nucleic Acid Conformation
  • RNA Ligase (ATP)
  • RNA, Catalytic
  • Sequence Analysis, RNA
  • Substrate Specificity
  • Templates, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 1074-5521

Digital Object Identifier (DOI)

  • 10.1016/s1074-5521(02)00110-2

PubMed ID

  • 11927255
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Additional Document Info

start page

  • 297

end page

  • 307

volume

  • 9

issue

  • 3

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