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Proteomic identification of palmitoylated proteins

Academic Article
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Overview

authors

  • Roth, A. F.
  • Wan, J. M.
  • Green, W. N.
  • Yates III, John
  • Davis, N. G.

publication date

  • October 2006

journal

  • Methods  Journal

abstract

  • A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae.

subject areas

  • Alkylation
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Palmitic Acid
  • Proteins
  • Proteome
  • Proteomics
  • Saccharomyces cerevisiae
  • Transferases
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Research

keywords

  • DHHC cysteine-rich domain
  • MudPIT
  • Saccharomyces cerevisiae
  • palmitoylation
  • protein acyl transferase
  • proteomics
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Identity

PubMed Central ID

  • PMC2806795

International Standard Serial Number (ISSN)

  • 1046-2023

Digital Object Identifier (DOI)

  • 10.1016/j.ymeth.2006.05.026

PubMed ID

  • 17012025
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Additional Document Info

start page

  • 135

end page

  • 142

volume

  • 40

issue

  • 2

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