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Thiazole orange as the fluorescent intercalator in a high resolution fid assay for determining DNA binding affinity and sequence selectivity of small molecules

Academic Article
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Overview

related to degree

  • Tse, Winston, Ph.D. in Chemistry, Scripps Research 1998 - 2003

authors

  • Boger, Dale
  • Tse, Winston

publication date

  • September 2001

journal

  • Bioorganic & Medicinal Chemistry  Journal

abstract

  • The viability of using thiazole orange as an alternative to ethidium bromide in a fluorescent intercalator displacement (FID) assay is explored by profiling the DNA binding affinity and sequence selectivity of netropsin. Utilizing a library of hairpin deoxyoligonucleotides containing all possible four base-pair sequences, the method provides a high resolution profile of the DNA binding properties of small molecules in a high throughput format.

subject areas

  • Anti-Bacterial Agents
  • Benzothiazoles
  • Binding, Competitive
  • DNA
  • Ethidium
  • Fluorescent Dyes
  • Gene Library
  • Intercalating Agents
  • Ligands
  • Microchemistry
  • Netropsin
  • Oligodeoxyribonucleotides
  • Quinolines
  • Spectrometry, Fluorescence
  • Thiazoles
  • Titrimetry
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Identity

International Standard Serial Number (ISSN)

  • 0968-0896

Digital Object Identifier (DOI)

  • 10.1016/s0968-0896(01)00243-7

PubMed ID

  • 11553493
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Additional Document Info

start page

  • 2511

end page

  • 2518

volume

  • 9

issue

  • 9

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