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Gene expression in scrapie - cloning a new scrapie-responsive gene and the identification of increased levels of seven other mRNA transcripts

Academic Article
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Overview

authors

  • Dandoy-Dron, F.
  • Guillo, F.
  • Benboudjema, L.
  • Deslys, J. P.
  • Lasmezas, Corinne
  • Dormont, D.
  • Tovey, M. G.
  • Dron, M.

publication date

  • 1998

journal

  • Journal of Biological Chemistry  Journal

abstract

  • To define genes associated with or responsible for the neurodegenerative changes observed in transmissible spongiform encephalopathies, we analyzed gene expression in scrapie-infected mouse brain using "mRNA differential display." The RNA transcripts of eight genes were increased 3-8-fold in the brains of scrapie-infected animals. Five of these genes have not previously been reported to exhibit increased expression in this disease: cathepsin S, the C1q B-chain of complement, apolipoprotein D, and two previously unidentified genes denominated scrapie-responsive gene (ScRG)-1 and ScRG-2, which are preferentially expressed in brain tissue. Increased expression of the three remaining genes, beta2 microglobulin, F4/80, and metallothionein II, has previously been reported to occur in experimental scrapie. Kinetic analysis revealed a concomitant increase in the levels of ScRG-1, cathepsin S, the C1q B-chain of complement, and beta2 microglobulin mRNA as well as glial fibrillary acidic protein and F4/80 transcripts, markers of astrocytosis and microglial activation, respectively. In contrast, the level of ScRG-2, apolipoprotein D, and metallothionein II mRNA was only increased at the terminal stage of the disease. ScRG-1 mRNA was found to be preferentially expressed in glial cells and to code for a short protein of 47 amino acids with a strong hydrophobic N-terminal region.

subject areas

  • Amino Acid Sequence
  • Animals
  • Apolipoproteins
  • Apolipoproteins D
  • Base Sequence
  • Brain
  • Cathepsins
  • Cloning, Molecular
  • Complement C1q
  • Gene Expression Regulation
  • Mice
  • Mice, Inbred C57BL
  • Microglia
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Scrapie
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.273.13.7691

PubMed ID

  • 9516475
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Additional Document Info

start page

  • 7691

end page

  • 7697

volume

  • 273

issue

  • 13

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