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Interleukin-6 selectively enhances the intracellular calcium response to NMDA in developing CNS neurons

Academic Article
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Overview

authors

  • Qiu, Z. H.
  • Parsons, K. L.
  • Gruol, Donna

publication date

  • October 1995

journal

  • Journal of Neuroscience  Journal

abstract

  • Increasing evidence supports a role for cytokines as chemical signals in the CNS, either under normal conditions or in the pathologic state. CNS levels of the cytokine interleukin-6 (IL-6) are known to be elevated in several diseases associated with developmental disorders and may contribute to the pathological state. To investigate the potential role of IL-6 in such disorders, neuronal effects of IL-6 were examined during development using an in vitro model system, cultured rat cerebellar granule neurons. The cultures were prepared from 8 d postnatal rat pups and exposed chronically to IL-6 (5 ng/ml) by addition to the culture medium. Neuronal effects of IL-6 were assessed by a comparison of calcium signals produced in control and IL-6 treated neurons by the glutamate receptor agonists NMDA and domoate and by K+ depolarization. IL-6 treatment significantly enhanced the response to NMDA and altered the developmental pattern of NMDA sensitivity, whereas only minor changes were observed for the response to domoate and K+. Reducing extracellular calcium and depleting intracellular stores significantly decreased the amplitude of the response to NMDA in control and IL-6 treated neurons. However, the IL-6 treated neurons were significantly more sensitive to these treatments than control neurons. These results suggest that elevated levels of IL-6 can significantly alter CNS neuron development and response to excitatory transmitters, and that IL-6 pretreatment selectively enhances the intracellular calcium responses to NMDA by altering the relative contribution of extracellular calcium influx and release of calcium from stores to the calcium signal.

subject areas

  • Animals
  • Calcium
  • Cells, Cultured
  • Cerebellum
  • Extracellular Space
  • Interleukin-6
  • Intracellular Membranes
  • Kainic Acid
  • N-Methylaspartate
  • Neurons
  • Potassium
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction
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Research

keywords

  • CYTOKINE
  • FURA-2
  • HIV GRANULE NEURONS
  • KAINATE RECEPTOR GROWTH FACTOR NEUROTROPIC FACTOR
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Identity

International Standard Serial Number (ISSN)

  • 0270-6474

PubMed ID

  • 7472429
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Additional Document Info

start page

  • 6688

end page

  • 6699

volume

  • 15

issue

  • 10

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