Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Novel beta-barrel fold in the nuclear magnetic resonance structure of the replicase nonstructural protein 1 from the severe acute respiratory syndrome coronavirus

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Almeida, M. S.
  • Johnson, M. A.
  • Herrmann, T.
  • Geralt, M.
  • Wuthrich, Kurt

publication date

  • April 2007

journal

  • Journal of Virology  Journal

abstract

  • The nonstructural protein 1 (nsp1) of the severe acute respiratory syndrome coronavirus has 179 residues and is the N-terminal cleavage product of the viral replicase polyprotein that mediates RNA replication and processing. The specific function of nsp1 is not known. Here we report the nuclear magnetic resonance structure of the nsp1 segment from residue 13 to 128, which represents a novel alpha/beta-fold formed by a mixed parallel/antiparallel six-stranded beta-barrel, an alpha-helix covering one opening of the barrel, and a 3(10)-helix alongside the barrel. We further characterized the full-length 179-residue protein and show that the polypeptide segments of residues 1 to 12 and 129 to 179 are flexibly disordered. The structure is analyzed in a search for possible correlations with the recently reported activity of nsp1 in the degradation of mRNA.

subject areas

  • Amino Acid Sequence
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Peptide Fragments
  • Protein Folding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • RNA Replicase
  • Recombinant Proteins
  • SARS Virus
  • Viral Nonstructural Proteins
scroll to property group menus

Identity

PubMed Central ID

  • PMC1866046

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.01939-06

PubMed ID

  • 17202208
scroll to property group menus

Additional Document Info

start page

  • 3151

end page

  • 3161

volume

  • 81

issue

  • 7

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support