Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Reconstitution of antiphage antibodies from L and H polypeptide chains and the formation of interspecies molecular hybrids

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Fougereau, M.
  • Olins, D. E.
  • Edelman, Gerald

publication date

  • 1964

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Reconstitution of phage-neutralizing activity was observed after admixture of separated L and H polypeptide chains of purified antibodies obtained from single sheep or guinea pigs and directed against f1 and f2 phages. Hybrid mixtures of complementary chains of antiphage antibodies and gamma-globulins of the same animal showed less activity than the homologous mixtures of chains from antibodies of the same specificity. 7S interspecies molecular hybrids could be formed between L or H chains of sheep gamma-globulins and the complementary chains of guinea pig gamma-globulins. In contrast to the results obtained within a single animal species, mixtures of L or H chains of antiphage antibodies from one species with the complementary chains of antibodies to the same phage from the other species showed slight or negligible potentiation of neutralizing activity.

subject areas

  • Animals
  • Antibodies
  • Bacteriophages
  • Electrophoresis
  • Guinea Pigs
  • Hybridization, Genetic
  • Neutralization Tests
  • Peptides
  • Research
  • Sheep
  • Ultracentrifugation
  • gamma-Globulins
scroll to property group menus

Identity

PubMed Central ID

  • PMC2137761

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.120.3.349

PubMed ID

  • 14207056
scroll to property group menus

Additional Document Info

start page

  • 349

end page

  • 358

volume

  • 120

issue

  • 3

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support