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Sequence of the spacer in the recombination signal sequence affects V(D)J rearrangement frequency and correlates with nonrandom V kappa usage in vivo

Academic Article
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Overview

authors

  • Nadel, B.
  • Tang, A.
  • Escuro, G.
  • Lugo, G.
  • Feeney, Ann

publication date

  • May 1998

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Functional variable (V), diversity (D), and joining (J) gene segments contribute unequally to the primary repertoire. One factor contributing to this nonrandom usage is the relative frequency with which the different gene segments rearrange. Variation from the consensus sequence in the heptamer and nonamer of the recombination signal sequence (RSS) is therefore considered a major factor affecting the relative representation of gene segments in the primary repertoire. In this study, we show that the sequence of the spacer is also a determinant factor contributing to the frequency of rearrangement. Moreover, the effect of the spacer on recombination rates of various human Vkappa gene segments in vitro correlates with their frequency of rearrangement in vivo in pre-B cells and with their representation in the peripheral repertoire.

subject areas

  • B-Lymphocytes
  • Base Sequence
  • Gene Rearrangement
  • Genes, Immunoglobulin
  • Humans
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Sorting Signals
  • Receptors, Antigen, T-Cell
  • Recombination, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.187.9.1495

PubMed ID

  • 9565641
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Additional Document Info

start page

  • 1495

end page

  • 1503

volume

  • 187

issue

  • 9

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