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High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

Academic Article
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Overview

authors

  • Medintz, I. L.
  • Paegel, Brian
  • Blazej, R. G.
  • Emrich, C. A.
  • Berti, L.
  • Scherer, J. R.
  • Mathies, R. A.

publication date

  • October 2001

journal

  • Electrophoresis  Journal

abstract

  • This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (microCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high-speed collection of sequencing and genotyping data from radially formatted microCAE devices. The concomitant development of a convenient energy-transfer cassette labeling chemistry allows sensitive multicolor labeling of any DNA genotyping or sequencing analyte. High-performance hereditary haemochromatosis and short tandem repeat genotyping assays are demonstrated on these devices along with rapid mitochondrial DNA sequence polymorphism analysis. Progress in supporting technology such as robotic fluid dispensing and batched data analysis is also presented. The ultimate goal is to develop a parallel analysis platform capable of integrated sample preparation and automated electrophoretic analysis with a throughput 10-100 times that of current technology.

subject areas

  • Electrophoresis, Capillary
  • Equipment Design
  • Fluorescent Dyes
  • Fluorometry
  • Genetic Techniques
  • Genome, Human
  • Genotype
  • HLA Antigens
  • Hemochromatosis
  • Histocompatibility Antigens Class I
  • Humans
  • Lasers
  • Membrane Proteins
  • Microchemistry
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA
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Research

keywords

  • DNA sequencing
  • capillary array electrophoresis
  • energy transfer fluorescent labels
  • genetic analysis
  • microchip
  • microfabricated genetic analysis devices
  • review
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Identity

International Standard Serial Number (ISSN)

  • 0173-0835

Digital Object Identifier (DOI)

  • 10.1002/1522-2683(200110)22:18<3845::aid-elps3845>3.0.co;2-0

PubMed ID

  • 11700713
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Additional Document Info

start page

  • 3845

end page

  • 3856

volume

  • 22

issue

  • 18

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