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Nonstimulatory peptides contribute to antigen-induced CD8-T cell receptor interaction at the immunological synapse

Academic Article
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Overview

authors

  • Yachi, Pia
  • Ampudia, J.
  • Gascoigne, Nicholas
  • Zal, T.

publication date

  • August 2005

journal

  • Nature Immunology  Journal

abstract

  • It is unclear if the interaction between CD8 and the T cell receptor (TCR)-CD3 complex is constitutive or antigen induced. Here, fluorescence resonance energy transfer microscopy between fluorescent chimeras of CD3zeta and CD8beta showed that this interaction was induced by antigen recognition in the immunological synapse. Nonstimulatory endogenous or exogenous peptides presented simultaneously with antigenic peptides increased the CD8-TCR interaction. This finding indicates that the interaction between the intracellular regions of a TCR-CD3 complex recognizing its cognate peptide-major histocompatibility complex (MHC) antigen, and CD8 (plus the kinase Lck), is enhanced by a noncognate CD8-MHC interaction. Thus, the interaction of CD8 with a nonstimulatory peptide-MHC complex helps mediate T cell recognition of antigen, improving the coreceptor function of CD8.

subject areas

  • Algorithms
  • Amino Acid Motifs
  • Antigens
  • Antigens, CD3
  • Antigens, CD8
  • Bacterial Proteins
  • CD8-Positive T-Lymphocytes
  • Cell Communication
  • Dose-Response Relationship, Drug
  • Down-Regulation
  • Flow Cytometry
  • Fluorescence Resonance Energy Transfer
  • Green Fluorescent Proteins
  • Humans
  • Hybridomas
  • Immunoblotting
  • Immunoprecipitation
  • Luminescent Proteins
  • Microscopy, Fluorescence
  • Peptides
  • Protein Binding
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • Time Factors
  • Tyrosine
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Identity

PubMed Central ID

  • PMC1352171

International Standard Serial Number (ISSN)

  • 1529-2908

Digital Object Identifier (DOI)

  • 10.1038/ni1220

PubMed ID

  • 15980863
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Additional Document Info

start page

  • 785

end page

  • 792

volume

  • 6

issue

  • 8

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