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Clearance of hepatitis B virus from the liver of transgenic mice by short hairpin RNAs

Academic Article
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Overview

authors

  • Uprichard, S. L.
  • Boyd, B.
  • Althage, A.
  • Chisari, Francis

publication date

  • January 2005

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Hepatitis B virus (HBV) causes acute and chronic hepatitis and hepatocellular carcinoma. Although a preventive vaccine is available, the therapeutic options for chronically infected patients are limited. It has been shown that RNA interference can prevent HBV gene expression and replication in vivo when HBV expression vectors are delivered simultaneously with small interfering RNA (siRNA) or siRNA expression constructs. However, the therapeutic potential of siRNAs to interrupt ongoing HBV replication in vivo has not been established. Here, we show that expression of HBV-specific siRNAs in the liver of HBV transgenic mice by recombinant adenoviruses can suppress preexisting HBV gene expression and replication to almost undetectable levels for at least 26 days. These results demonstrate that efficiently delivered siRNAs should be able to silence HBV in chronically infected patients.

subject areas

  • Adenoviridae
  • Animals
  • Chronic Disease
  • Genetic Vectors
  • Hepatitis B
  • Hepatitis B virus
  • Hepatitis, Chronic
  • Liver
  • Mice
  • Mice, Transgenic
  • RNA, Small Interfering
  • RNA, Viral
  • Time
  • Virus Replication
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Research

keywords

  • RNA interference
  • adenovirus vector
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Identity

PubMed Central ID

  • PMC545555

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0409028102

PubMed ID

  • 15640346
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Additional Document Info

start page

  • 773

end page

  • 778

volume

  • 102

issue

  • 3

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