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Cd22 is a recycling receptor that can shuttle cargo between the cell surface and endosomal compartments of b cells

Academic Article
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Overview

authors

  • O'Reilly, M. K.
  • Tian, H.
  • Paulson, James

publication date

  • February 2011

journal

  • Journal of Immunology  Journal

abstract

  • CD22 is a member of the sialic acid-binding Ig-like lectin (Siglec) family that is known to be a regulator of B cell signaling. Its B cell-specific expression makes it an attractive target for immunotoxin-mediated B cell depletion therapy for the treatment of B cell lymphomas and autoimmune diseases. Although CD22 is well documented to be an endocytic receptor, it is believed that after internalization, it is targeted for degradation. We show in this study that CD22 is instead constitutively recycled to the cell surface. We also find that glycan ligand-based cargo is released from CD22 and accumulates intracellularly as CD22 recycles between the cell surface and endosomal compartments. In contrast, Abs to CD22 do not accumulate but remain bound to CD22 and recycle to the cell surface. The results have implications for development of agents that target CD22 as an endocytic receptor for delivery of cytotoxic cargo to B cells.

subject areas

  • Animals
  • B-Lymphocyte Subsets
  • Burkitt Lymphoma
  • CHO Cells
  • Cell Line, Tumor
  • Cell Membrane
  • Cells, Cultured
  • Cricetinae
  • Cricetulus
  • Drug Delivery Systems
  • Endocytosis
  • Endosomes
  • Humans
  • Intracellular Fluid
  • Ligands
  • Lymphocyte Depletion
  • Lymphoma, B-Cell
  • Mice
  • Mice, Inbred C57BL
  • Protein Transport
  • Sialic Acid Binding Ig-like Lectin 2
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Identity

PubMed Central ID

  • PMC3024466

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.1003005

PubMed ID

  • 21178016
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Additional Document Info

start page

  • 1554

end page

  • 1563

volume

  • 186

issue

  • 3

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