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Role of gut macrophages in mice orally contaminated with scrapie or BSE

Academic Article
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Overview

authors

  • Maignien, T.
  • Shakwed, M.
  • Calvo, P.
  • Marce, D.
  • Sales, N.
  • Fattal, E.
  • Deslys, J. P.
  • Couvreur, P.
  • Lasmezas, Corinne

publication date

  • July 2005

journal

  • International Journal of Pharmaceutics  Journal

abstract

  • While there is a growing consensus on the understanding of the propagation pathways after oral infection of transmissible spongiform encephalopathy (TSE) agents and even if the central role of follicular dendritic cells is identified, little is known about the key players in the first steps of the infection and about the site of the disease development. We investigated the role of gut macrophages, which are capable of capturing aggregates of the prion protein. PLGA particles containing clodronate were designed in order to be orally administered and to target Peyer's patches for inducing gut-associated macrophages suicide in mice. Mice were subsequently infected with scrapie or BSE by the oral route. It was found that the efficacy of macrophage suppression in the Peyer's patches correlated well with an earlier appearance of PrPres in these formations and with a higher amount of PrPres at a later stage of the infection. Thus, the capture of infectious particles that have crossed the epithelial gut barrier and their elimination by macrophages seems to be a key event to restrict the amount of agent initiating the infection.

subject areas

  • Animals
  • Cattle
  • Chemistry, Physical
  • Clodronic Acid
  • Digestive System
  • Drug Compounding
  • Encephalopathy, Bovine Spongiform
  • Fluorescent Dyes
  • Immunohistochemistry
  • Lactic Acid
  • Macrophages
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Electron, Scanning
  • Microspheres
  • Particle Size
  • Peyer's Patches
  • Physicochemical Phenomena
  • Polyglycolic Acid
  • Polymers
  • Rhodamines
  • Scrapie
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Research

keywords

  • Peyer's patches
  • clodronate
  • intestinal uptake
  • macrophage-suicide technique
  • nano- and microparticles
  • transmissible spongiform encephalopaties
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Identity

International Standard Serial Number (ISSN)

  • 0378-5173

Digital Object Identifier (DOI)

  • 10.1016/j.ijpharm.2005.02.042

PubMed ID

  • 15964722
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Additional Document Info

start page

  • 293

end page

  • 304

volume

  • 298

issue

  • 2

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