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Localization of Smc5/6 to centromeres and telomeres requires heterochromatin and SUMO, respectively

Academic Article
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Overview

authors

  • Pebernard, S.
  • Schaffer, L.
  • Campbell, D.
  • Head, Steve
  • Boddy, Michael

publication date

  • November 2008

journal

  • EMBO Journal  Journal

abstract

  • The Smc5/6 holocomplex executes key functions in genome maintenance that include ensuring the faithful segregation of chromosomes at mitosis and facilitating critical DNA repair pathways. Smc5/6 is essential for viability and therefore, dissecting its chromosome segregation and DNA repair roles has been challenging. We have identified distinct epigenetic and post-translational modifications that delineate roles for fission yeast Smc5/6 in centromere function, versus replication fork-associated DNA repair. We monitored Smc5/6 subnuclear and genomic localization in response to different replicative stresses, using fluorescence microscopy and chromatin immunoprecipitation (ChIP)-on-chip methods. Following hydroxyurea treatment, and during an unperturbed S phase, Smc5/6 is transiently enriched at the heterochromatic outer repeats of centromeres in an H3-K9 methylation-dependent manner. In contrast, methyl methanesulphonate treatment induces the accumulation of Smc5/6 at subtelomeres, in an Nse2 SUMO ligase-dependent, but H3-K9 methylation-independent manner. Finally, we determine that Smc5/6 loads at all genomic tDNAs, a phenomenon that requires intact consensus TFIIIC-binding sites in the tDNAs.

subject areas

  • Carrier Proteins
  • Cell Cycle Proteins
  • Cell Nucleus
  • Centromere
  • Chromosomal Proteins, Non-Histone
  • DNA Replication
  • DNA, Fungal
  • Heterochromatin
  • Hydroxyurea
  • Methyl Methanesulfonate
  • Mutagens
  • Nucleic Acid Synthesis Inhibitors
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Small Ubiquitin-Related Modifier Proteins
  • Telomere
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Research

keywords

  • SMC5
  • SUMO
  • heterochromatin
  • recombination
  • tDNA
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Identity

PubMed Central ID

  • PMC2585169

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1038/emboj.2008.220

PubMed ID

  • 18923417
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Additional Document Info

start page

  • 3011

end page

  • 3023

volume

  • 27

issue

  • 22

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