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A T cell receptor-specific blockade of positive selection

Academic Article
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Overview

authors

  • Baldwin, Kristin
  • Reay, P. A.
  • Wu, L.
  • Farr, A.
  • Davis, M. M.

publication date

  • January 1999

journal

  • Journal of Experimental Medicine  Journal

abstract

  • To investigate the influence of endogenous peptides on the developmental processes that occur during thymocyte selection, we have used monoclonal antibodies that preferentially recognize the major histocompatibility complex (MHC) molecule I-Ek when it is bound to the moth cytochrome c peptide (88-103). One of these antibodies (G35) specifically blocks the positive selection of transgenic thymocytes expressing a T cell receptor that is reactive to this peptide- MHC complex. Furthermore, G35 does not block the differentiation of transgenic T cells bearing receptors for a different I-Ek-peptide complex. This antibody recognizes a subset of endogenous I-Ek-peptide complexes found on a significant fraction of thymic antigen-presenting cells, including cortical and medullary epithelial cells. The sensitivity of G35 to minor alterations in peptide sequence suggests that the thymic peptide-MHC complexes that mediate the positive selection of a particular class II MHC-restricted thymocyte are structurally related to the complexes that can activate it in the periphery.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Cytochrome c Group
  • Flow Cytometry
  • Histocompatibility Antigens Class II
  • Immunohistochemistry
  • Insect Proteins
  • Lymph Nodes
  • Major Histocompatibility Complex
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Peptide Fragments
  • Receptors, Antigen, T-Cell
  • Spleen
  • T-Lymphocytes
  • Thymus Gland
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Research

keywords

  • CD4(+) T cell
  • monoclonal antibodies
  • peptides
  • thymic selection
  • thymocyte
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Identity

PubMed Central ID

  • PMC1887687

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.189.1.13

PubMed ID

  • 9874560
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Additional Document Info

start page

  • 13

end page

  • 23

volume

  • 189

issue

  • 1

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