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Mass spectral analysis in proteomics

Academic Article
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Overview

authors

  • Yates III, John

publication date

  • 2004

journal

  • Annual Review of Biophysics and Biomolecular Structure  Journal

abstract

  • Mass spectrometry provides key tools for the analysis of proteins. New types of mass spectrometers that provide enhanced capability to discover protein identities and perform improved proteomic experiments are discussed. Handling the complex mixtures of peptides and proteins generated from protein complexes and whole cells requires multidimensional separations; several forms of separation are discussed. Applications of mass spectrometry-based approaches for contemporary proteomic analyses are described.

subject areas

  • Chromatography
  • Complex Mixtures
  • Electrophoresis
  • Mass Spectrometry
  • Proteins
  • Proteome
  • Proteomics
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Research

keywords

  • ESI
  • MALDI
  • multidimensional separations
  • tandem mass spectrometry
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Identity

International Standard Serial Number (ISSN)

  • 1056-8700

Digital Object Identifier (DOI)

  • 10.1146/annurev.biophys.33.111502.082538

PubMed ID

  • 15139815
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Additional Document Info

start page

  • 297

end page

  • 316

volume

  • 33

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