Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Overexpression of interleukin-10 by activated t lymphocytes inhibits atherosclerosis in ldl receptor-deficient mice by altering lymphocyte and macrophage phenotypes

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Pinderski, L. J.
  • Fischbein, M. P.
  • Subbanagounder, G.
  • Fishbein, M. C.
  • Kubo, N.
  • Cheroutre, H.
  • Curtiss, Linda
  • Berliner, J. A.
  • Boisvert, W. A.

publication date

  • May 2002

journal

  • Circulation Research  Journal

abstract

  • Previous studies demonstrated that interleukin-10 (IL-10) overexpression decreases formation of early fatty-streak lesions in mice independent of lipoprotein levels. The present studies, using bone marrow transplantation, demonstrate that overexpression of IL-10 by T cells inhibits advanced atherosclerotic lesions in LDL receptor-null mice fed an atherogenic diet. In mice receiving bone marrow from the IL-10 transgenic mice compared with those receiving wild-type marrow, there was a 47% decrease in lesion size and a marked decrease in lesion complexity with an 80% reduction in the necrotic core. Accumulation of cholesterol and phospholipid oxidation products in the aorta was decreased by 50% to 80%, unrelated to plasma lipid or IL-10 levels. Our studies also provide insight into the mechanism of the IL-10-mediated decrease in lesion size. Although a strong influence toward a Th1 phenotype has previously been demonstrated in atherosclerotic models, T lymphocytes in the IL-10 transgenic (Tg) group revealed a marked shift to a Th2 phenotype, with decreased IFN-gamma production and an increase in IL-10. Evaluation of specific immunoglobulin subclasses demonstrated a preponderance of IgG(1) isotype, characteristic of a Th2 influence on B cell immunoglobulin class-switching in the IL-10 Tg group. A major finding of these studies was altered monocyte/macrophage function in the IL-10 Tg group. Monocytes showed a decrease in activation resulting in decreased expression of IFN-gamma. Furthermore, macrophage foam cells within lesions of the IL-10 Tg group exhibited markedly decreased apoptosis. These studies demonstrate that T lymphocyte IL-10 can influence the function of other immune cells to reduce the development of advanced atherosclerotic lesions in mice.

subject areas

  • Animals
  • Aorta
  • Arteriosclerosis
  • Bone Marrow Transplantation
  • Flow Cytometry
  • Immunoglobulins
  • Interleukin-10
  • Lipids
  • Lipoproteins
  • Lymphocyte Activation
  • Macrophages
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Monocytes
  • Phenotype
  • Receptors, LDL
  • T-Lymphocytes, Helper-Inducer
  • Th2 Cells
scroll to property group menus

Research

keywords

  • B lymphocytes
  • LDL receptor
  • antibodies
  • apoptosis
  • cytokine
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0009-7330

Digital Object Identifier (DOI)

  • 10.1161/01.res.0000018941.10726.fa

PubMed ID

  • 12039795
scroll to property group menus

Additional Document Info

start page

  • 1064

end page

  • 1071

volume

  • 90

issue

  • 10

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support