Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

A novel approach for in vivo measurement of mouse red cell redox status

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Xu, X. L.
  • von Lohneysen, K.
  • Soldau, K.
  • Noack, D.
  • Vu, A.
  • Friedman, Jeffrey

publication date

  • September 2011

journal

  • Blood  Journal

abstract

  • Maintenance of a reducing redox balance is a critical physiologic function of red cells (RBC) that can be perturbed in variety of RBC pathologies. Here we describe a new approach to evaluate in vivo RBC redox status using a redox sensitive GFP (roGFP2) sensor under control of a β-globin mini-promoter, directing expression specifically to erythroid cells. RoGFP2 expressing RBCs demonstrate ratiometric and reversible shifts in fluorescence on exposure to oxidants and reductants. We demonstrate that roGFP2 expressing RBC can be used to monitor thiol redox status during in vitro phenylhydrazine treatment and over the course of in vivo RBC aging, where a shift to a more oxidized state is observed in older cells. Thus, roGFP2 transgenic mice are a new and versatile tool that can be used to probe how RBC redox status responds in the context of drug therapy, physiologic stressors and pathologic states.

subject areas

  • Animals
  • Blood Chemical Analysis
  • Cell Aging
  • Cell Tracking
  • Erythrocyte Indices
  • Erythrocytes
  • Female
  • Green Fluorescent Proteins
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Oxidation-Reduction
scroll to property group menus

Identity

PubMed Central ID

  • PMC3186341

International Standard Serial Number (ISSN)

  • 0006-4971

Digital Object Identifier (DOI)

  • 10.1182/blood-2011-03-342113

PubMed ID

  • 21832281
scroll to property group menus

Additional Document Info

start page

  • 3694

end page

  • 3697

volume

  • 118

issue

  • 13

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support