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Isolation and chemical characterization of two structurally and functionally distinct forms of botrocetin, the platelet coagglutinin isolated from the venom of Bothrops jararaca

Academic Article
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Overview

authors

  • Fujimura, Y.
  • Titani, K.
  • Usami, Y.
  • Suzuki, M.
  • Oyama, R.
  • Matsui, T.
  • Fukui, H.
  • Sugimoto, M.
  • Ruggeri, Zaverio

publication date

  • February 1991

journal

  • Biochemistry  Journal

abstract

  • Two distinct forms of botrocetin, the von Willebrand factor (vWF)-dependent platelet coagglutinin isolated from the venom of the snake Bothrops jararaca, were purified and characterized structurally and functionally. The apparent molecular mass of the one-chain botrocetin was 28 kDa before and 32 kDa after reduction of disulfide bonds, while that of the two-chain botrocetin was 27 kDa before and 15/14.5 kDa after reduction. Amino acid composition of the two species revealed a similar high content of potentially acidic residues (greater than 60 Asx and Glx residues/molecule) but significant differences in the content of Cys and Phe residues. The NH2-terminal sequence of the one-chain botrocetin was Ile-Ile/Val-Ser-Pro-Pro-Val-Cys-Gly-Asn-Glu-. Two constituent polypeptides of the two-chain botrocetin showed similar but different NH2-terminal sequences, distinct from that of the one-chain species: (alpha) Asp-Cys-Pro-Ser-Gly-Trp-Ser-Ser-Tyr-Glu- and (beta) Asp-Cys-Pro-Pro-Asp-Trp-Ser-Ser-Tyr-Glu-. The carbohydrate content of both species was less than 2% of the total mass, and the pI was 4.0-4.1 for the one-chain species, and 4.6, 5.3-5.4, and 7.7-7.8 for the two-chain species. No free sulfhydryl group was detected in each species. Both types of botrocetin were resistant to proteolysis at neutral pH. Incubation of 125I-labeled one-chain botrocetin with the crude venom solution resulted in no detectable structural change. On a weight basis, the two-chain botrocetin was 34 times more active than the one-chain form in promoting vWF binding to platelets.(ABSTRACT TRUNCATED AT 250 WORDS)

subject areas

  • Amino Acid Sequence
  • Amino Acids
  • Animals
  • Blood Platelets
  • Carbohydrates
  • Crotalid Venoms
  • Hemagglutinins
  • Humans
  • Kinetics
  • Macromolecular Substances
  • Molecular Sequence Data
  • Molecular Weight
  • Sequence Homology, Nucleic Acid
  • Snakes
  • Sulfhydryl Compounds
  • von Willebrand Factor
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Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi00221a032

PubMed ID

  • 1993206
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Additional Document Info

start page

  • 1957

end page

  • 1964

volume

  • 30

issue

  • 7

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