Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Okada, M.
  • Cheeseman, I. M.
  • Hori, T.
  • Okawa, K.
  • McLeod, I. X.
  • Yates III, John
  • Desai, A.
  • Fukagawa, T.

publication date

  • May 2006

journal

  • Nature Cell Biology  Journal

abstract

  • In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. Here, we isolated a multi-subunit complex, which includes the established inner kinetochore components CENP-H and CENP-I, and nine other proteins, from both human and chicken cells. Our analysis of these proteins demonstrates that the CENP-H-I complex can be divided into three functional sub-complexes, each of which is required for faithful chromosome segregation. Interestingly, newly expressed CENP-A is not efficiently incorporated into centromeres in knockout mutants of a subclass of CENP-H-I complex proteins, indicating that the CENP-H-I complex may function, in part, as a marker directing CENP-A deposition to centromeres.

subject areas

  • Animals
  • Autoantigens
  • Cell Cycle
  • Cells, Cultured
  • Centromere
  • Chickens
  • Chromosomal Proteins, Non-Histone
  • Chromosome Segregation
  • DNA-Binding Proteins
  • HeLa Cells
  • Humans
  • Models, Biological
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins
  • Protein Binding
  • Protein Transport
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1465-7392

Digital Object Identifier (DOI)

  • 10.1038/ncb1396

PubMed ID

  • 16622420
scroll to property group menus

Additional Document Info

start page

  • 446

end page

  • 457

volume

  • 8

issue

  • 5

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support