Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Differential arrangements of conserved building blocks among homologs of the rad50/mre11 DNA repair protein complex

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • de Jager, M.
  • Trujillo, K. M.
  • Sung, P.
  • Hopfner, K. P.
  • Carney, J. P.
  • Tainer, John
  • Connelly, J. C.
  • Leach, D. R. F.
  • Kanaar, R.
  • Wyman, C.

publication date

  • June 2004

journal

  • Journal of Molecular Biology  Journal

abstract

  • Structural maintenance of chromosomes (SMC) proteins have diverse cellular functions including chromosome segregation, condensation and DNA repair. They are grouped based on a conserved set of distinct structural motifs. All SMC proteins are predicted to have a bipartite ATPase domain that is separated by a long region predicted to form a coiled coil. Recent structural data on a variety of SMC proteins shows them to be arranged as long intramolecular coiled coils with a globular ATPase at one end. SMC proteins function in pairs as heterodimers or as homodimers often in complexes with other proteins. We expect the arrangement of the SMC protein domains in complex assemblies to have important implications for their diverse functions. We used scanning force microscopy imaging to determine the architecture of human, Saccharomyces cerevisiae, and Pyrococcus furiosus Rad50/Mre11, Escherichia coli SbcCD, and S.cerevisiae SMC1/SMC3 cohesin SMC complexes. Two distinct architectural arrangements are described, based on the way their components were connected. The eukaryotic complexes were similar to each other and differed from their prokaryotic and archaeal homologs. These similarities and differences are discussed with respect to their diverse mechanistic roles in chromosome metabolism.

subject areas

  • Amino Acid Sequence
  • DNA Repair
  • DNA-Binding Proteins
  • Humans
  • Molecular Sequence Data
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Species Specificity
scroll to property group menus

Research

keywords

  • DNA metabolism
  • SbcCD
  • protein architecture
  • scanning force microscopy (SFM)
  • structural maintenance of chromosomes (SMQ)
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/j.jmb.2004.04.014

PubMed ID

  • 15165861
scroll to property group menus

Additional Document Info

start page

  • 937

end page

  • 949

volume

  • 339

issue

  • 4

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support