recent publications

• academic article

  • Rader, C., Segal, D. J., Shabat, D. Carlos F. Barbas III (1964-2014): Visionary at the interface of chemistry and biology ACS Chemical Biology  2014 9:1645-1646  DOI:10.1021/cb5005993  PMID:25123302
  • Segal, D. J., Crotty, J. W., Bhakta, M. S., Barbas, C. F., Horton, N. C. Structure of aart, a designed six-finger zinc finger peptide, bound to DNA Journal of Molecular Biology  2006 363:405-421  DOI:10.1016/j.jmb.2006.08.016  PMID:16963084
  • Eberhardy, S. R., Goncalves, J., Coelho, S., Segal, D. J., Berkhout, B., Barbas, C. F. Inhibition of human immunodeficiency virus type 1 replication with artificial transcription factors targeting the highly conserved primer-binding site Journal of Virology  2006 80:2873-2883  DOI:10.1128/jvi.80.6.2873-2883.2006  PMID:16501096
  • Dreier, B., Fuller, R. P., Segal, D. J., Lund, C. V., Blancafort, P., Huber, A., Koksch, B., Barbas, C. F. Development of zinc finger domains for recognition of the 5'-CNN-3' family DNA sequences and their use in the construction of artificial transcription factors Journal of Biological Chemistry  2005 280:35588-35597  DOI:10.1074/jbc.M506654200  PMID:16107335
  • Crotty, J. W., Etzkorn, C., Barbas, C. F., 3rd, Segal, D. J., Horton, N. C. Crystallization and preliminary x-ray crystallographic analysis of aart, a designed six-finger zinc-finger peptide, bound to DNA Acta Crystallographica Section F-Structural Biology and Crystallization Communications  2005 61:573-576  DOI:10.1107/s1744309105014363  PMID:16511099  PMCID:PMC1952332
  • Blancafort, P., Segal, D. J., Barbas, C. F. Designing transcription factor architectures for drug discovery Molecular Pharmacology  2004 66:1361-1371  DOI:10.1124/mol.104.002758.  PMID:15340042
  • Segal, D. J., Goncalves, J., Eberhardy, S., Swan, C. H., Torbett, B. E., Li, X. L., Barbas, C. F. Attenuation of HIV-1 replication in primary human cells with a designed zinc finger transcription factor Journal of Biological Chemistry  2004 279:14509-14519  DOI:10.1074/jbc.M400349200  PMID:14734553
  • Tan, W. J., Zhu, K., Segal, D. J., Barbas, C. F., Chow, S. A. Fusion proteins consisting of human immunodeficiency virus type 1 integrase and the designed polydactyl zinc finger protein e2c direct integration of viral DNA into specific sites Journal of Virology  2004 78:1301-1313  DOI:10.1128/jvi.78.3.1301-1313.2004  PMID:14722285
  • Segal, D. J., Stege, J. T., Barbas, C. F. Zinc fingers and a green thumb: Manipulating gene expression in plants Current Opinion in Plant Biology  2003 6:163-168  DOI:10.1016/s1369-5266(03)00007-4  PMID:12667874
  • Segal, D. J., Beerli, R. R., Blancafort, P., Dreier, B., Effertz, K., Huber, A., Koksch, B., Lund, C. V., Magnenat, L., Valente, D., Barbas, C. F. Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins Biochemistry  2003 42:2137-2148  DOI:10.1021/bi026806o  PMID:12590603
  • Segal, D. J., Barbas, C. F. Custom DNA-binding proteins come of age: Polydactyl zinc-finger proteins Current Opinion in Biotechnology  2001 12:632-637  DOI:10.1016/s0958-1669(01)00272-5  PMID:11849947
  • Dreier, B., Beerli, R. R., Segal, D. J., Flippin, J. D., Barbas, C. F. Development of zinc finger domains for recognition of the 5 '-ann-3 ' family of DNA sequences and their use in the construction of artificial transcription factors Journal of Biological Chemistry  2001 276:29466-29478  DOI:10.1074/jbc.M102604200  PMID:11340073
  • Dreier, B., Segal, D. J., Barbas, C. F. Insights into the molecular recognition of the 5 '-gnn-3 ' family of DNA sequences by zinc finger domains Journal of Molecular Biology  2000 303:489-502  DOI:10.1006/jmbi.2000.4133  PMID:11054286
  • Segal, D. J., Barbas, C. F. Design of novel sequence-specific DNA-binding proteins Current Opinion in Chemical Biology  2000 4:34-39  DOI:10.1016/s1367-5931(99)00048-4  PMID:10679372
  • Barbas, C. F., Rader, C., Segal, D. J., List, B., Turner, J. M. From catalytic asymmetric synthesis to the transcriptional regulation of genes: in vivo and in vitro evolution of proteins Advances in Protein Chemistry  2000 55:317-366  DOI:10.1016/S0065-3233(01)55008-1  PMID:11050938
  • Segal, D. J., Dreier, B., Beerli, R. R., Barbas, C. F. Toward controlling gene expression at will: Selection and design of zinc finger domains recognizing each of the 5 '-gnn-3 ' DNA target sequences Proceedings of the National Academy of Sciences of the United States of America  1999 96:2758-2763  DOI:10.1073/pnas.96.6.2758  PMID:10077584
  • Beerli, R. R., Segal, D. J., Dreier, B., Barbas, C. F. Toward controlling gene expression at will: Specific regulation of the erbb-2/her-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks Proceedings of the National Academy of Sciences of the United States of America  1998 95:14628-14633  DOI:10.1073/pnas.95.25.14628  PMID:9843940
  • Liu, Q., Segal, D. J., Ghiara, J. B., Barbas, C. F. Design of polydactyl zinc-finger proteins for unique addressing within complex genomes Proceedings of the National Academy of Sciences of the United States of America  1997 94:5525-5530  DOI:10.1073/pnas.94.11.5525  PMID:9159105